Skip to main content

Evaluation of Arsenic Trioxide Potential for Lung Cancer Treatment: ASSessment of Apoptotic Mechanisms and Oxidative Damage

Thumbnail for Evaluation of Arsenic Trioxide Potential for Lung Cancer Treatment: ASSessment of Apoptotic Mechanisms and Oxidative Damage
View
@ Clark Atlanta University

Walker, Alice M Stevens, Jacqueline J Ndebele, Kenneth Tchounwou, Paul B

Description

Abstract: Background: Lung cancer is one of the most lethal and common cancers in the world, causing up to 3 million deaths annually. The chemotherapeutic drugs that have been used in treating lung cancer include cisplatinpemetrexed, cisplastin-gencitabinoe, carboplatin-pacitaxel and crizotinib. Arsenic trioxide (ATO) has been used in the treatment of acute promyelocytic leukemia. However, its effects on lung cancer are not known. We hypothesize that ATO may also have a bioactivity against lung cancer, and its mechanisms of action may involve apoptosis, DNA damage and changes in stress-related proteins in lung cancer cells. Methods: To test the above stated hypothesis, lung carcinoma (A549) cells were used as the test model. The effects of ATO were examined by performing 6-diamidine-2 phenylindole (DAPI) nuclear staining for morphological characterization of apoptosis, flow cytometry analysis for early apoptosis, and western blot analysis for stressrelated proteins (Hsp70 and cfos) and apoptotic protein expressions. Also, the single cell gel electrophoresis (Comet) assay was used to evaluate the genotoxic effect. Results: ATO-induced apoptosis was evidenced by chromatin condensation and formation of apoptotic bodies as revealed by DAPI nuclear staining. Cell shrinkage and membrane blebbing were observed at 4 and 6 ug/ml of ATO. Data from the western blot analysis revealed a significant dose-dependent increase (p less than 0.05) in the Hisp 70, caspase 3 and p53 protein expression, and a significant (p less than 0.05) decrease in the cfos, and bol-2 protein expression at 4 and 6 g/ml of ATO. There was a slight decrease in cytochrome...

Record Contributed By

Clark Atlanta University

Record Harvested From

Digital Library of Georgia

Keywords

  1. African Americans
  2. Clark Atlanta University
  3. Education (Higher)
  4. Georgia

Related Content

Association of Epithelial Mesenchymal Transition with prostate and breast health disparities Block Decompositions and Applications of Generalized Reflexive Matrices Muscadine Grape Skin Extract Induces an Unfolded Protein Response-Mediated Autophagy in Prostate Cancer Cells: A TMTBased Quantitative Proteomic Analysis Selective Small-Chemical Inhibitors of Protein Arginine Methyltransferase 5 with Anti-Lung Cancer Activity Lignin-Coated Cellulose Nanocrystals as Promising Nucleating Agent for Poly(Lcetic Acid). Issues of Effective Field Theories with Resonances 174G/C Polymorphism in the Interleukin-6 Promoter Is Differently Associated with Prostate Cancer Incidence Depending on Race Photoionization of Fe7+ from the ground and metastable states An investigation into the efficacy of gender-specific sexual assault education within a university setting Classroom Learning Assessment Interpretation in Journalism and Mass Communication in Higher Education: A Focus on Andragogy in Radio/ Television/ Film Production Modifications and News in the World Methodology of Violin/Viola Training in the Beginning of the XXIst Century Internalized racism and its effects on African American women Birth order: A social work examination of predicting resilience The impact of parental incarceration Intra-Tumoral Delivery of Functional ID4 Protein via PCL/ Maltodextrin Nano-Particle Inhibits Prostate Cancer Growth Chlamydial Infection in Vitamin D Receptor Knockout Mice is More Intense and Prolonged than in Wild-type Mice Off Center Effect on the Photoabsorption Spectrum of the Xe@C60 Endohedral Fullerene Social environment, Physical environment and life expectancy of Black women K-shell Photoionization of Atomic Cl The African American Presence in Physics
Download JSON